Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17751] to PPCEL/PREPL - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free
    See all PPCEL/PREPL primary antibodies

  • Description

    Rabbit monoclonal [EPR17751] to PPCEL/PREPL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251361 is the carrier-free version of ab202064.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/10000 dilution
    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Immunoprecipitation - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

    ab202064&

    44; the same antibody clone in a different buffer formulation.

    >PPCEL/PREPL was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell extract with ab202064 at 1/40.> Western blot was performed using ab202064 at 1/2000.> Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.> Lane 1: MCF7 whole cell lysate at 10ug.> Lane 2: Immunoprecipitate of MCF7 whole cell lysate using ab202064.> Lane 3: Immunoprecipitate of MCF7 whole cell lysate using ab172730 (Rabbit monoclonal IgG).
  • Flow Cytometry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Flow Cytometry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryo fibroblast) cells labeling PPCEL/PREPL with ab202064 at 1/150 (red) compared with a rabbit monoclonal IgG isotype control (ab172730) (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody (blue)). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Immunocytochemistry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Immunocytochemistry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human neuroblastoma from bone marrow cells) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on SH-SY5Y cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/500 (red).
    The negative controls are as follows:-
    -ve control 1 - ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution.
    -ve control 2. - Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
  • Immunocytochemistry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Immunocytochemistry - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    This data was developed using ab202064, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling PPCEL/PREPL with ab202064 at 1/1000, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Confocal image showing cytoplasmic staining on MCF-7 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/500 dilution (red). The negative controls are as follows:- -ve control 1: ab202064 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/500 dilution.
  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus)) lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/1000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Western blot - Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    All lanes : Anti-PPCEL/PREPL antibody [EPR17751] (ab202064) at 1/5000 dilution

    Lane 1 : A673 (Human muscle Ewing's Sarcoma cell line) lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line ) lysate
    Lane 3 : L6 (Rat skeletal muscle cell line) lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 72 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab202064, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)
    Anti-PPCEL/PREPL antibody [EPR17751] - BSA and Azide free (ab251361)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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