Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13086] to PPA2 - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPA2 antibody [EPR13086] - BSA and Azide free
    See all PPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13086] to PPA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250259 is the carrier-free version of ab180859. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250259 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Anti-PPA2 antibody [EPR13086] (ab180859) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified) + 293T whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?



    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)

    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with purified ab180859 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)

    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

    ab180859 (purified) at 1/80 immunoprecipitating PPA2 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg).

    Lane 2 (+): ab180859 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180859 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Western blot - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U-87 MG cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 38 kDa



    This data was developed using ab180859, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    This data was developed using ab180859, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    This data was developed using ab180859, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Immunoprecipitation - Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    This data was developed using ab180859, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180859 at a diltuion of 1/10.
  • Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)
    Anti-PPA2 antibody [EPR13086] - BSA and Azide free (ab250259)

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