Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-PPA2 antibody [EPR13086] (ab180859)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13086] to PPA2
Suitable for: IHC-P, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-PPA2 antibody [EPR13086]
See all PPA2 primary antibodies
Description

Rabbit monoclonal [EPR13086] to PPA2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human PPA2 aa 1-100. The exact sequence is proprietary.
Database link: Q9H2U2
Positive control
- WB: Human fetal kidney mouse and rat heart tissue lysates and 293T, U87-MG and HepG2 cell lysates. IHC-P: Human brain and kidney tissues. IP: HepG2 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13086
Isotype

IgG
Research areas

Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified)

Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 38 kDa
Observed band size: 35 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

Anti-PPA2 antibody [EPR13086] (ab180859) at 1/10000 dilution (purified) + HepG2 whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 38 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (purified) + 293T whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 38 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with purified ab180859 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

ab180859 (purified) at 1/80 immunoprecipitating PPA2 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10µg)
Lane 2 (+): ab180859 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180859 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-PPA2 antibody [EPR13086] (ab180859)

All lanes : Anti-PPA2 antibody [EPR13086] (ab180859) at 1/1000 dilution (unpurified)

Lane 1 : Human fetal kidney lysate
Lane 2 : 293T lysate
Lane 3 : U87-MG lysate
Lane 4 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPA2 antibody [EPR13086] (ab180859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling PPA2 with unpurified ab180859 at a dilution of 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-PPA2 antibody [EPR13086] (ab180859)

Western blot analysis of immunoprecipitation pellet from HepG2 lysate immunoprecipitated using unpurified ab180859 at a diltuion of 1/10.
Anti-PPA2 antibody [EPR13086] (ab180859)

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