Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PcMab-47] to PODXL - BSA and Azide free
  • Suitable for: Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PODXL antibody [PcMab-47] - BSA and Azide free
    See all PODXL primary antibodies

  • Description

    Mouse monoclonal [PcMab-47] to PODXL - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Caco-2 cells. Wild-type HAP1 cells.

  • General notes

    ab264549 is the carrier-free version of ab264542. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab264549 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)
    Flow Cytometry - Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)

    This data was developed using ab264542 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of parental HAP1 (Wildtype control human chronic myelogenous leukemia near-haploid cell line, Right) / PODXL KO HAP1 (Left) cells labelling PODXL with ab264542 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Flow Cytometry - Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)
    Flow Cytometry - Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)

    This data was developed using ab264542 the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of MDA-MB-468 (Human breast adenocarcinoma epithelial cell, Left) / Caco-2 (Human colorectal adenocarcinoma epithelial cell, Right) cells labelling PODXL with ab264542 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

    Low expression control: MDA-MB-468. (PMID: 28384052).

    Gated on viable cells.

  • Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)
    Anti-PODXL antibody [PcMab-47] - BSA and Azide free (ab264549)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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