Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
![Anti-PLVAP/PV-1 antibody [174/2] (ab81719)](https://www.abcam.com/ps/products/81/ab81719/Images/PLVAP-Primary-antibodies-ab81719-1.gif "Anti-PLVAP/PV-1 antibody [174/2] (ab81719)")
Key features and details
- Mouse monoclonal [174/2] to PLVAP/PV-1
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PLVAP/PV-1 antibody [174/2]
See all PLVAP/PV-1 primary antibodies -
Description
Mouse monoclonal [174/2] to PLVAP/PV-1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Tissue, cells or virus corresponding to Human PLVAP/PV-1.
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Positive control
- Human tonsil cells.
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General notes
Previously labelled as PLVAP.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab81719 is 0.2µM filtered. -
Clonality
Monoclonal -
Clone number
174/2 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)
ab81719 at 1/50 dilution staining PLVAP/PV-1 in human tonsil by Immunofluorescence.
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![Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)](https://www.abcam.com/ps/products/81/ab81719/Images/PLVAP-Primary-antibodies-ab81719-2.jpg)
Flow Cytometry - Anti-PLVAP/PV-1 antibody [174/2] (ab81719)Overlay histogram showing HL60 cells stained with ab81719 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81719, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
