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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)

Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8381] to Plunc - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Plunc antibody [EPR8381] - BSA and Azide free
    See all Plunc primary antibodies
  • Description

    Rabbit monoclonal [EPR8381] to Plunc - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248364 is the carrier-free version of ab131163. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 3.90 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8381
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    Western blot - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    All lanes : Anti-Plunc antibody [EPR8381] (ab131163) at 1/1000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : PC3 cell lysate
    Lane 3 : Human lung squamous cell cancer lysate
    Lane 4 : Human fetal lung tissue lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab131163, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    Flow Cytometry - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    This data was developed using ab131163, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells labelling Plunc (red) or a rabbit IgG negative control (green) with ab131163 antibody at 1/10 dilution.
  • OI-RD Scanning - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    OI-RD Scanning - Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    This data was developed using ab131163, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)
    Anti-Plunc antibody [EPR8381] - BSA and Azide free (ab248364)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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