Anti-Plexin B1 antibody (ab39717)
Key features and details
- Rabbit polyclonal to Plexin B1
- Suitable for: IHC-FoFr, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Plexin B1 antibody
See all Plexin B1 primary antibodies -
Description
Rabbit polyclonal to Plexin B1 -
Host species
Rabbit -
Specificity
PLexin B1 is readily cleaved at the intra- extra- cellular boundary (circa aa.1305/1306) likely giving rise to the several different band sizes routinely observed in WB, depending on the isoform that has been cleaved. -
Tested applications
Suitable for: IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Plexin B1.
Read Abcam's proprietary immunogen policy (Peptide available as ab41062.) -
Positive control
- WB: Mouse and rat liver tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-FoFr 1/1000.WB Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).Notes IHC-FoFr
1/1000.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 233 kDa).Target
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Function
Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. -
Tissue specificity
Highly expressed in fetal kidney, and at slightly lower levels in fetal brain, lung and liver. -
Sequence similarities
Belongs to the plexin family.
Contains 3 IPT/TIG domains.
Contains 1 Sema domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues by ERBB2 and MET upon SEMA4D binding.
Proteolytic processing favors heterodimerization with PLXNB2 and SEMA4D binding. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5364 Human
- Entrez Gene: 235611 Mouse
- Entrez Gene: 316009 Rat
- Omim: 601053 Human
- SwissProt: O43157 Human
- SwissProt: Q8CJH3 Mouse
- Unigene: 476209 Human
- Unigene: 53862 Mouse
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Alternative names
- KIAA0407 antibody
- Plexin 5 antibody
- Plexin B1 antibody
see all
Images
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Western blot - Anti-Plexin B1 antibody (ab39717)All lanes : Anti-Plexin B1 antibody (ab39717) at 1 µg/ml
Lane 1 : Mouse liver tissue lysate
Lane 2 : Rat liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab39717 has been referenced in 3 publications.
- Gerstmann K et al. A balance of noncanonical Semaphorin signaling from the cerebrospinal fluid regulates apical cell dynamics during corticogenesis. Sci Adv 8:eabo4552 (2022). PubMed: 36399562
- Wu JH et al. Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy. EMBO Mol Med 12:e10154 (2020). PubMed: 31943789
- Daviaud N et al. Impaired cortical neurogenesis in plexin-B1 and -B2 double deletion mutant. Dev Neurobiol 76:882-99 (2016). PubMed: 26579598
Images
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Western blot - Anti-Plexin B1 antibody (ab39717)All lanes : Anti-Plexin B1 antibody (ab39717) at 2 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Pancreas (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 233 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 155 kDa. We are unsure as to the identity of these extra bands.
We believe that the band observed at 171 kDa corresponds to a cleaved form of Plexin B1. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Plexin B1 antibody (ab39717) This image is courtesy of Sophie Pezet, CNRS, Paris, France
Immunohistochemistical detection of Plexin B1 with antibody ab39717 on PFA perfusion-fixed rat brain sections. Primary antibody ab39717 was diluted 1/1000 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 conjugated (1/1000). Immunostaining obtained in the hypothalamus, next to the rat brain ventricle. Immunostaining was performed using the `free floating` technique, using direct fluorescence. The image shows staining observed in glial cells. Some staining was observed on cells bordering the ventricle, but no other type of cells such as neurons. This is consistent with the literature where immunoreactivity is published in glial cells and neurons & as suggestted by several studies, as by Ito Y (EMBO Rep. 2006 Jul7(7):704-9).
