Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKN2 antibody [EPR5490] (ab138514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5490] to PKN2
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PKN2 antibody [EPR5490]
    See all PKN2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5490] to PKN2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PKN2 aa 900-1000. The exact sequence is proprietary.

  • Positive control

    • WB: HCT116, HEK-293T, Jurkat, HeLa, and HepG2 (ab7900) cell lysates. ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5490

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

    Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 2 : PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 112 kDa
    Observed band size: 125 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 125 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab138514 Anti-PKN2 antibody [EPR5490] (ab138514) was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PKN2 antibody [EPR5490] (ab138514)
    Immunocytochemistry/ Immunofluorescence - Anti-PKN2 antibody [EPR5490] (ab138514)
    Immunofluorescent analysis of HeLa cells labelling PKN2 with ab138514 at 1/100 dilution.
  • Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PKN2 knockout HeLa cell lysate
    Lane 3 : HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 112 kDa
    Observed band size: 112 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    Western blot - Anti-PKN2 antibody [EPR5490] (ab138514)
    All lanes : Anti-PKN2 antibody [EPR5490] (ab138514) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 112 kDa
    Observed band size: 112 kDa

  • Anti-PKN2 antibody [EPR5490] (ab138514)
    Anti-PKN2 antibody [EPR5490] (ab138514)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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