Anti-PITX3/PTX3 antibody (ab30734)
Key features and details
- Rabbit polyclonal to PITX3/PTX3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PITX3/PTX3 antibody
See all PITX3/PTX3 primary antibodies -
Description
Rabbit polyclonal to PITX3/PTX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
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Positive control
- WB: Y79, U87-MG, and HEK-293 whole cell lysates.
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General notes
Western blot blocking was performed with 5% milk.This product was previously labelled as PITX3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB 1/250. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). Abcam recommends using 5% milk as the blocking agent.Notes WB
1/250. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa). Abcam recommends using 5% milk as the blocking agent.Target
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Function
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle. -
Tissue specificity
Highly expressed in developing eye lens. -
Involvement in disease
Defects in PITX3 are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
Defects in PITX3 are a cause of anterior segment mesenchymal dysgenesis (ASMD) [MIM:107250]; also known as anterior segment ocular dysgenesis (ASOD). ASMD consists of a range of developmental defects in structures at the front of the eye, resulting from abnormal migration or differentiation of the neural crest derived mesenchymal cells that give rise to the cornea, iris, and other components of the anterior chamber during eye development. Mature anterior segment anomalies are associated with an increased risk of glaucoma and corneal opacity. Conditions falling within the phenotypic spectrum include aniridia, posterior embryotoxon, Axenfeld anomaly, Reiger anomaly/syndrome, Peters anomaly, and iridogoniodysgenesis.
Defects in PITX3 are the cause of cataract posterior polar type 4 (CTPP4) [MIM:610623]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity. Some patients affected by cataract posterior polar type 4 can present a severe phenotype including microphthalmia and neurological dysfunction. -
Sequence similarities
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5309 Human
- Entrez Gene: 18742 Mouse
- Entrez Gene: 29609 Rat
- Omim: 602669 Human
- SwissProt: O75364 Human
- SwissProt: Q5VZL2 Human
- SwissProt: O35160 Mouse
- SwissProt: P81062 Rat
see all -
Alternative names
- Homeobox protein PITX 3 antibody
- Homeobox protein PITX3 antibody
- MGC12766 antibody
see all
Images
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Western blot - Anti-PITX3/PTX3 antibody (ab30734)All lanes : Anti-PITX3/PTX3 antibody (ab30734) at 1 µg/ml (5% Milk block)
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (10)
ab30734 has been referenced in 10 publications.
- Liu Y et al. Pentraxin 3 promotes the osteoblastic differentiation of MC3T3-E1 cells through the PI3K/Akt signaling pathway. Biosci Rep 40:N/A (2020). PubMed: 32436939
- Márquez-Valadez B et al. The Systemic Administration of the Histamine H1 Receptor Antagonist/Inverse Agonist Chlorpheniramine to Pregnant Rats Impairs the Development of Nigro-Striatal Dopaminergic Neurons. Front Neurosci 13:360 (2019). PubMed: 31040765
- Raina A et al. Neuronal Trans-differentiation by Transcription Factors Ascl1 and Nurr1: Induction of a Dopaminergic Neurotransmitter Phenotype in Cortical GABAergic Neurons. Mol Neurobiol N/A:N/A (2019). PubMed: 31317490
- Chabrat A et al. Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons. Stem Cells Int 2019:2945435 (2019). PubMed: 31236114
- Mahajani S et al. Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors. Cell Death Dis 10:898 (2019). PubMed: 31776327
- Gracia-Rubio I et al. Maternal Separation Impairs Cocaine-Induced Behavioural Sensitization in Adolescent Mice. PLoS One 11:e0167483 (2016). PubMed: 27936186
- García-Pérez D et al. Morphine administration modulates expression of Argonaute 2 and dopamine-related transcription factors involved in midbrain dopaminergic neurons function. Br J Pharmacol 168:1889-901 (2013). PubMed: 23215787
- Volpicelli F et al. Direct regulation of Pitx3 expression by Nurr1 in culture and in developing mouse midbrain. PLoS One 7:e30661 (2012). WB ; Mouse . PubMed: 22363463
- Nguyen HN et al. LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress. Cell Stem Cell 8:267-80 (2011). WB, IHC-P ; Human . PubMed: 21362567
- Ning H et al. MicroRNA regulation of neuron-like differentiation of adipose tissue-derived stem cells. Differentiation 78:253-9 (2009). WB ; Rat . PubMed: 19695767
Images
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Western blot - Anti-PITX3/PTX3 antibody (ab30734)All lanes : Anti-PITX3/PTX3 antibody (ab30734) at 1 µg/ml (5% Milk block)
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
