Anti-Pirh2 antibody [EPR14980] (ab189247)
![Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-389681-recombinant-anti-pirh2-antibody-epr14980-western-blot-human-lysate.jpg "Anti-Pirh2 antibody [EPR14980] (ab189247)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14980] to Pirh2
- Suitable for: WB, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Pirh2 antibody [EPR14980]
See all Pirh2 primary antibodies -
Description
Rabbit monoclonal [EPR14980] to Pirh2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB RatHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Daudi, 293T, LnCaP, HeLa, C6, Raw 264.7 and NIH/3T3 cell lysates. IHC-P: Mouse liver tissue. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14980 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RCHY1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDaLanes 1-4: Merged signal (red and green). Green - ab189247 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.
ab189247 Recombinant Anti-Pirh2 antibody [EPR14980] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189247 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-224602-anti-pirh2-antibody-epr14980-western-blot.jpg)
Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : LnCaP cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
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![Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-224600-anti-pirh2-antibody-epr14980-western-blot.jpg)
Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-224601-anti-pirh2-antibody-epr14980-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (ab189247)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Pirh2 with ab189247 at 1/50 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-224599-anti-pirh2-antibody-epr14980-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (ab189247)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells labeling Pirh2 with ab189247 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with DAPI (blue).
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![Anti-Pirh2 antibody [EPR14980] (ab189247)](https://www.abcam.com/ps/products/189/ab189247/Images/ab189247-6-benefits-of-recombinant-antibodies.png)
Anti-Pirh2 antibody [EPR14980] (ab189247)
