Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pirh2 antibody [EPR14980] (ab189247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14980] to Pirh2
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pirh2 antibody [EPR14980]
    See all Pirh2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14980] to Pirh2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi, 293T, LnCaP, HeLa, C6, Raw 264.7 and NIH/3T3 cell lysates. IHC-P: Mouse liver tissue. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : RCHY1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab189247 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab189247 Recombinant Anti-Pirh2 antibody [EPR14980] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189247 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : LnCaP cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247)
    All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw 264.7 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (ab189247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (ab189247)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Pirh2 with ab189247 at 1/50 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (ab189247)
    Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (ab189247)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells labeling Pirh2 with ab189247 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with DAPI (blue).

  • Anti-Pirh2 antibody [EPR14980] (ab189247)
    Anti-Pirh2 antibody [EPR14980] (ab189247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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