Anti-Piccolo antibody [6H9-B6] (ab101654)
![Anti-Piccolo antibody [6H9-B6] (ab101654)](https://www.abcam.com/ps/products/101/ab101654/Images/ab101654-310904-anti-piccolo-antibody-6h9-b6-immunofluorescence.jpg "Anti-Piccolo antibody [6H9-B6] (ab101654)")
Key features and details
- Mouse monoclonal [6H9-B6] to Piccolo
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Piccolo antibody [6H9-B6]
See all Piccolo primary antibodies -
Description
Mouse monoclonal [6H9-B6] to Piccolo -
Host species
Mouse -
Specificity
No cross reactivity to Bassoon. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length protein corresponding to Mouse Piccolo.
Database link: Q9QYX7 -
Positive control
- WB: Rat and mouse brain tissue lysate. ICC/IF: SK-N-BE cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
6H9-B6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Piccolo antibody [6H9-B6] (ab101654)
SK-N-BE cells labeling Piccolo using ab101654 (green) at 1/100 dilution in ICC/IF. Cells were fixed using 4% formaldehyde for 15 minutes at room temperature. Primary antibody was incubated for 1 hour at room temperature. Secondary antibody was a goat anti-mouse ATTO 488 at 1/100 dilution for 1 hour at room temperature. Counterstained with Phalloidin Texas Red F-actin stain. Nuclei were stained with DAPI (blue).
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![Western blot - Anti-Piccolo antibody [6H9-B6] (ab101654)](https://www.abcam.com/ps/products/101/ab101654/Images/ab101654-199655-anti-piccolo-antibody-6h9-b6-western-blot.jpg)
Western blot - Anti-Piccolo antibody [6H9-B6] (ab101654)All lanes : Anti-Piccolo antibody [6H9-B6] (ab101654) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Predicted band size: 567 kDa
