Anti-HBP1 (phospho S402) antibody (ab73339)
Key features and details
- Rabbit polyclonal to HBP1 (phospho S402)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HBP1 (phospho S402) antibody
See all HBP1 primary antibodies -
Description
Rabbit polyclonal to HBP1 (phospho S402) -
Host species
Rabbit -
Specificity
ab73339 detects endogenous levels of HBP1 only when phosphorylated at serine 402 (Human) or serine 404 (Mouse). -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic phosphopeptide derived from human HBP1 around the phosphorylation site of serine 402 (CGSPPG)
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Positive control
- Extracts from A549 cells treated with PMA (125ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab73339 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HBP1 (phospho S402) antibody (ab73339) at 1/500 dilution
Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins)
Lane 2 : Extracts from A549 cells treated with PMA (125ng/ml, 30 mins) with immunising phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa