Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23919-31] to PHF19 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free
    See all PHF19 primary antibodies

  • Description

    Rabbit monoclonal [EPR23919-31] to PHF19 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, WB, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, U-87 MG, HeLa and HEK-293T whole cell lysates. ICC: K562 cells. Flow cyt: K562 and HeLa cells. IP: K562 whole cell lysate.

  • General notes

    ab277641 is the carrier-free version of ab259947. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277641is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Western blot - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Lane 1 : (ab259974) at 1/1000 dilution
    Lanes 2-4 : Anti-PHF19 antibody [EPR23919-31] (ab259947) at 1/1000 dilution

    Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa
    Observed band size: 22,70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259947, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 15 seconds; Lane 2: 3 minutes; Lanes 3-4: 147 seconds.

     

  • Immunoprecipitation - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Immunoprecipitation - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

    This data was developed using ab259947, the same antibody clone in a different buffer formulation.

    PHF19 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab259947 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259947 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 ug

    Lane 2: ab259947 IP in K-562 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259947 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

     

  • Immunocytochemistry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Immunocytochemistry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

    This data was developed using ab259947, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K-562  (human chronic myelogenous leukemia lymphoblast) cells labelling PHF19 with ab259947 at 1/100 dilution (4.94 ug/ml), followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and cytoplasmic staining in K562 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Flow Cytometry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

    This data was developed using ab259947, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling PHF19 with ab259947 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Flow Cytometry - Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

    This data was developed using ab259947, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast) cells labelling PHF19 with ab259947 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)
    Anti-PHF19 antibody [EPR23919-31] - BSA and Azide free (ab277641)

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