Anti-PHF10 antibody (ab154637)
Key features and details
- Rabbit polyclonal to PHF10
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PHF10 antibody -
Description
Rabbit polyclonal to PHF10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseHuman
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 243-459 of Human PHF10 (UniProt ID: Q8WUB8).
-
Positive control
- WB: HEK-293T and HeLa whole cell lysates; Mouse colon lysate. ICC: HeLa cells. IHC-P: HeLa xenograft.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PHF10 antibody (ab154637)All lanes : Anti-PHF10 antibody (ab154637) at 1/500 dilution
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : PHF10 knockout HEK-293 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HeLa (Nuclear prepped) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa (possible non-specific binding)Lanes 1 - 4: Merged signal (red and green). Green - ab154637 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154637 was shown to react with PHF10 in wild-type HEK-293 cells in Western blot with loss of signal observed in PHF10 knockout sample. Wild-type HEK-293 and PHF10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154637 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
Immunocytochemistry/ Immunofluorescence - Anti-PHF10 antibody (ab154637)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling PHF10 with ab154637 at a 1/500 dilution. Alpha-tubulin filaments were labeled (red) at 1/2000. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF10 antibody (ab154637)Immunohistochemical analysis of paraffin embedded Hela xenograft labeling PHF10 with ab154637 at a 1/500 dilution. -
Western blot - Anti-PHF10 antibody (ab154637)Anti-PHF10 antibody (ab154637) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 56 kDa
10% SDS PAGE -
Western blot - Anti-PHF10 antibody (ab154637)Anti-PHF10 antibody (ab154637) at 1/1000 dilution + mouse colon lysate/ extract at 50 µg
Predicted band size: 56 kDa
10 % SDS-PAGE