Anti-PHAPI2 / APRIL antibody (ab4224)
Key features and details
- Goat polyclonal to PHAPI2 / APRIL
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-PHAPI2 / APRIL antibody
See all PHAPI2 / APRIL primary antibodies -
Description
Goat polyclonal to PHAPI2 / APRIL -
Host species
Goat -
Specificity
This antibody does NOT recognize the TNF family member also known as APRIL - LocusLink Number 8741 -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide: KRKRETDDEGEDD, corresponding to C terminal amino acids 237-249 of Human PHAPI2/ APRIL.
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Positive control
- Human Tonsil lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)All lanes : Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml
Lane 1 : Human tonsil lysate
Lane 2 : Rat spleen lysate
Lane 3 : Pig spleen lysate
Lysates/proteins at 35 mg/ml per lane.
Predicted band size: 29 kDa
Observed band size: 28-30 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)Anti-PHAPI2 / APRIL antibody (ab4224) at 0.1 µg/ml + NIH3T3 cell lysate at 35 µg
Predicted band size: 29 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)ab4224 at a concentration of 1µ g/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30µ g total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL). ab4224 at a concentration of 1 µg/ml, staining ~30 kDa PHAP I2 in human lymph node lysate (RIPA bufer, 30 µg total proteion per lane) by western blot (primary incubated for 1 hour, detected by ECL). -
Western blot - Anti-PHAPI2 / APRIL antibody (ab4224)Anti-PHAPI2 / APRIL antibody (ab4224) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 29 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAPI2 / APRIL antibody (ab4224)ab4224 (3.8µg/ml) staining of paraffin embedded Human Breast tissue following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows nuclear staining in lobular cells.
