Anti-PGP9.5 antibody (ab10404)
Key features and details
- Rabbit polyclonal to PGP9.5
- Suitable for: IHC-Fr, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PGP9.5 antibody
See all PGP9.5 primary antibodies -
Description
Rabbit polyclonal to PGP9.5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Horse
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Immunogen
Synthetic peptide:
ASSEDTLLKDAAKVCR
, corresponding to amino acids 176 - 191 of Human PGP9.5. -
General notes
Serum may become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGP9.5 antibody (ab10404)Anti-PGP9.5 antibody (ab10404) at 1/1000 dilution + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 26.8 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 70 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody (ab10404)ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
µ m thick coronal sections; see protocol link for detailed description). Image taken with a 20x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
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Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody (ab10404)ab10404 at a dilution of 1/2000, staining PGP9.5 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).
