Anti-PGK1 antibody (ab233135)
Key features and details
- Rabbit polyclonal to PGK1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PGK1 antibody
See all PGK1 primary antibodies -
Description
Rabbit polyclonal to PGK1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Chimpanzee, Cynomolgus monkey, Chinese hamster, Orangutan
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human PGK1 aa 1 to the C-terminus. Two N-terminal tags. Expressed in E.coli.
Database link: P00558 -
Positive control
- WB: Recombinant human PGK1 protein; HeLa, HepG2 and HEK-293T cell lysates. IHC-P: Human cerebrum, colorectal cancer, glioma, liver and kidney tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PGK1 antibody (ab233135)Anti-PGK1 antibody (ab233135) at 2 µg/ml + Recombinant human PGK1 protein
Predicted band size: 45 kDa
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Western blot - Anti-PGK1 antibody (ab233135)All lanes : Anti-PGK1 antibody (ab233135) at 1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 45 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab233135)Formalin-fixed, paraffin-embedded human cerebrum tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab233135)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab233135)Formalin-fixed, paraffin-embedded human glioma tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab233135)Formalin-fixed, paraffin-embedded human liver tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGK1 antibody (ab233135)Formalin-fixed, paraffin-embedded human kidney tissue stained for PGK1 using ab233135 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-PGK1 antibody (ab233135)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PGK1 (green) using ab233135 at 20 μg/ml in ICC/IF. FITC labeling.
