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Signal Transduction Metabolism Energy Metabolism

Anti-PFKFB2 antibody (ab70175)

Anti-PFKFB2 antibody (ab70175)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PFKFB2
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PFKFB2 antibody
    See all PFKFB2 primary antibodies
  • Description

    Rabbit polyclonal to PFKFB2
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total PFKFB2 protein.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    A synthesized peptide derived from the N-terminal of human PFKFB2.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-PFKFB2 antibody (ab70175)
    Western blot - Anti-PFKFB2 antibody (ab70175)
    All lanes : Anti-PFKFB2 antibody (ab70175) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    Various other bands are detected, from 26 to over 117kDa.
  • Western blot - Anti-PFKFB2 antibody (ab70175)
    Western blot - Anti-PFKFB2 antibody (ab70175) This image is courtesy of an anonymous Abreview
    Anti-PFKFB2 antibody (ab70175) at 1/500 dilution (in 5% Marvel TBS-T for 24 hours at 22°C) + Whole cell lysate of mouse NIH 3T£ cells at 25 µg

    Secondary
    An HRP-conjugated Sheep anti-rabbit monoclonal at 1/5000 dilution

    Predicted band size: 58 kDa


    Exposure time: 8 minutes


    Blocking Step: 5% BSA for 1 hour at 22°C

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: ICC/IF, IHC-P, WB

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    Applications: IP, WB

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