Anti-PFAS antibody (ab251740)
Key features and details
- Rabbit polyclonal to PFAS
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PFAS antibody
See all PFAS primary antibodies -
Description
Rabbit polyclonal to PFAS -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human PFAS aa 650-800.
Database link: O15067 -
Positive control
- IHC-P: Human endometrium, tonsil, testis, lymph node and kidney tissues. ICC/IF: A431 cells. WB: NIH/3T3, NBT-II, RT4 and U-251 MG whole cell lysates; human liver and tonsil lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PFAS antibody (ab251740)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PFAS (green) using ab251740 at 4 µg/ml in ICC/IF.
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Western blot - Anti-PFAS antibody (ab251740)All lanes : Anti-PFAS antibody (ab251740) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 145 kDa
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Western blot - Anti-PFAS antibody (ab251740)All lanes : Anti-PFAS antibody (ab251740) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 145 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human kidney tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human lymph node tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human testis tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human tonsil tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human endometrium tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFAS antibody (ab251740)Paraffin-embedded human skeletal muscle tissue stained for PFAS using ab251740 at 1/20 dilution in immunohistochemical analysis. No positivity, as expected.