Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [mAbcam 28144] to Pericentrin - BSA and Azide free
  • Suitable for: ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free
    See all Pericentrin primary antibodies

  • Description

    Mouse monoclonal [mAbcam 28144] to Pericentrin - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • ICC: HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab256363 is the carrier-free version of ab28144. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256363 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)
    Immunocytochemistry - Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)

    This data was developed using the same antibody clone in a different buffer formulation (ab28144).

    Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
    antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.

    Confocal image showing centrosome staining in HeLa cells.

  • Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)
    Anti-Pericentrin antibody [mAbcam 28144] - BSA and Azide free (ab256363)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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