Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 16, 2021

Anti-Perforin antibody [EPR22829-42] (ab256453)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22829-42] to Perforin
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Perforin antibody [EPR22829-42]
See all Perforin primary antibodies
Description

Rabbit monoclonal [EPR22829-42] to Perforin
Host species

Rabbit

Tested Applications & Species

Application	Species
IP	Mouse
WB	Mouse Rat Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-tagged mouse PRF1 recombinant protein 10 ng (21-554); Mouse primary spleen (treated with 10 ng/ml human IL-2 for 3 days) whole cell lysate; rat spleen lysate. IP: Mouse spleen lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22829-42
Isotype

IgG
Research areas

Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)

Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution + Rat spleen lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 6 seconds.
Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] (ab256453)

PRF1 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab256453 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256453 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1: Mouse spleen lysate 10ug

Lane 2: ab256453 IP in Mouse spleen lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256453 in Mouse spleen lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)

All lanes : Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution

Lane 1 : Untreated mouse primary spleen whole cell lysate
Lane 2 : Mouse primary spleen treated with 10 ng/ml human IL-2 for 3 days, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 26 seconds.
Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)

Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution + His-tagged mouse PRF1 recombinant protein (21-554), 10 ng

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The recombinant protein was expressed from a E. coli inclusion body.

Exposure time: 8 seconds.
Anti-Perforin antibody [EPR22829-42] (ab256453)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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