Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Perforin antibody [EPR22829-42] (ab256453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22829-42] to Perforin
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Perforin antibody [EPR22829-42]
    See all Perforin primary antibodies

  • Description

    Rabbit monoclonal [EPR22829-42] to Perforin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse PRF1 recombinant protein 10 ng (21-554); Mouse primary spleen (treated with 10 ng/ml human IL-2 for 3 days) whole cell lysate; rat spleen lysate. IP: Mouse spleen lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22829-42

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution + Rat spleen lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Immunoprecipitation - Anti-Perforin antibody [EPR22829-42] (ab256453)

    PRF1 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab256453 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256453 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse spleen lysate 10ug

    Lane 2: ab256453 IP in Mouse spleen lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256453 in Mouse spleen lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

     

  • Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    All lanes : Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution

    Lane 1 : Untreated mouse primary spleen whole cell lysate
    Lane 2 : Mouse primary spleen treated with 10 ng/ml human IL-2 for 3 days, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.   

    Exposure time: 26 seconds.

  • Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Western blot - Anti-Perforin antibody [EPR22829-42] (ab256453)
    Anti-Perforin antibody [EPR22829-42] (ab256453) at 1/1000 dilution + His-tagged mouse PRF1 recombinant protein (21-554), 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The recombinant protein was expressed from a E. coli inclusion body. 

    Exposure time: 8 seconds.

  • Anti-Perforin antibody [EPR22829-42] (ab256453)
    Anti-Perforin antibody [EPR22829-42] (ab256453)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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