Anti-PDZK1 antibody (ab121248)
Key features and details
- Rabbit polyclonal to PDZK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PDZK1 antibody
See all PDZK1 primary antibodies -
Description
Rabbit polyclonal to PDZK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Chinese hamster
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Immunogen
Recombinant fragment: MTSTFNPREC KLSKQEGQNY GFFLRIEKDT EGHLVRVVEK CSPAEKAGLQ DGDRVLRING VFVDKEEHMQ VVDLVRKSGN SVTLLVLDGD SYEKAV, corresponding to amino acids 1-96 of Human PDZK1
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Positive control
- Human, mouse and rat liver lysate; Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDZK1 antibody (ab121248)Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate -
Immunocytochemistry/ Immunofluorescence - Anti-PDZK1 antibody (ab121248)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121248 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Western blot - Anti-PDZK1 antibody (ab121248)All lanes : Anti-PDZK1 antibody (ab121248)
Lane 1 : Caco-2 cells transfected with control siRNA
Lane 2 : Caco-2 cells transfected with target specific siRNA probe #1
Predicted band size: 57 kDaLoading control: Anti-GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDZK1 antibody (ab121248)Immunohistochemical staining of paraffin embedded Human kidney, using 1/150 ab121248, shows strong cytoplasmic positivity in cells in tubules.
