Anti-ZIP7 antibody (ab254566)
Key features and details
- Rabbit polyclonal to ZIP7
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ZIP7 antibody -
Description
Rabbit polyclonal to ZIP7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ZIP7 aa 20-70. The exact sequence is proprietary. 17 amino acids in length.
Database link: Q92504 -
Positive control
- WB: Mouse, rat and human brain tissue lysates. NIH/3T3, HEK-293, A549, HeLa, HepG2, K562, MCF7 and SK-N-SH cell lysate. IHC-P: Human brain tissue. ICC/IF: Human brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ZIP7 antibody (ab254566)All lanes : Anti-ZIP7 antibody (ab254566) at 1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : MCF7 cell lysate
Lane 7 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 50 kDa
-
Western blot - Anti-ZIP7 antibody (ab254566)All lanes : Anti-ZIP7 antibody (ab254566) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 50 kDa
-
Western blot - Anti-ZIP7 antibody (ab254566)Lane 1 : Anti-ZIP7 antibody (ab254566) at 0.5 µg/ml
Lane 2 : Anti-ZIP7 antibody (ab254566) at 1 µg/ml
All lanes : Rat brain tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 50 kDa
-
Western blot - Anti-ZIP7 antibody (ab254566)Anti-ZIP7 antibody (ab254566) at 0.5 µg/ml + Mouse brain tissue lysate
Predicted band size: 50 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZIP7 antibody (ab254566)Immunohistochemical analysis of human brain tissue labeling ZIP7 with ab254566 at 0.5µg/ml.
-
Immunocytochemistry/ Immunofluorescence - Anti-ZIP7 antibody (ab254566)Immunocytochemistry/Immunofluorescence analysis of human brain tissue labeling ZIP7 with ab254566 at 20µg/ml.