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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Assembly

Anti-PDPK1 antibody [3H3D9] (ab234064)

Anti-PDPK1 antibody [3H3D9] (ab234064)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [3H3D9] to PDPK1
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PDPK1 antibody [3H3D9]
    See all PDPK1 primary antibodies
  • Description

    Mouse monoclonal [3H3D9] to PDPK1
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human PDPK1 aa 457-556. (Expressed in E.coli).
    Database link: O15530

  • Positive control

    • WB: MCF7, HeLa, and U937 cell lysates; Human PDPK1 (aa 457-556) recombinant protein; PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    3H3D9
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases

Images

  • Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : U937 (human histiocytic lymphoma cell line) cell lysate

    Predicted band size: 63 kDa

  • Flow Cytometry - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Flow Cytometry - Anti-PDPK1 antibody [3H3D9] (ab234064)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PDPK1 with ab234064 at 1/200 dilution (green) compared with a negative control (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Immunocytochemistry/ Immunofluorescence - Anti-PDPK1 antibody [3H3D9] (ab234064)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PDPK1 (green) using ab234064 at 1/200 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution + Human PDPK1 (aa 457-556) recombinant protein

    Predicted band size: 63 kDa



    Expected MW is 37.9 kDa.

  • Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    Western blot - Anti-PDPK1 antibody [3H3D9] (ab234064)
    All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 63 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PDPK1 antibody [3H3D9] (ab234064)

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