Anti-PDPK1 antibody [3H3D9] (ab234064)
Key features and details
- Mouse monoclonal [3H3D9] to PDPK1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PDPK1 antibody [3H3D9]
See all PDPK1 primary antibodies -
Description
Mouse monoclonal [3H3D9] to PDPK1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human PDPK1 aa 457-556. (Expressed in E.coli).
Database link: O15530 -
Positive control
- WB: MCF7, HeLa, and U937 cell lysates; Human PDPK1 (aa 457-556) recombinant protein; PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3H3D9 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : U937 (human histiocytic lymphoma cell line) cell lysate
Predicted band size: 63 kDa
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PDPK1 with ab234064 at 1/200 dilution (green) compared with a negative control (red).
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PDPK1 (green) using ab234064 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution + Human PDPK1 (aa 457-556) recombinant protein
Predicted band size: 63 kDaExpected MW is 37.9 kDa.
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All lanes : Anti-PDPK1 antibody [3H3D9] (ab234064) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : PDPK1 (aa 457-556)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 63 kDa