Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13015] to PDK1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDK1 antibody [EPR13015] - BSA and Azide free
    See all PDK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13015] to PDK1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250175 is the carrier-free version of ab180171 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250175 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
    Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)

    This data was developed using ab180171, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)

    Lane 3: Human fetal heart tissue lysate (20 µg)

    Lane 4: LnCaP cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab180171 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180171 was shown to specifically react with Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab180171 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
    Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
    All lanes : Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171) at 1/10000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : LNCaP cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 49 kDa



    This data was developed using ab180171, the same antibody clone in a different buffer formulation.

     

  • Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
    Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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