Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
![Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)](https://www.abcam.com/ps/products/250/ab250175/Images/ab250175-403405-anti-pdk1-antibody-epr13015-bsa-and-azide-free-western-blot.jpg "Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13015] to PDK1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PDK1 antibody [EPR13015] - BSA and Azide free
See all PDK1 primary antibodies -
Description
Rabbit monoclonal [EPR13015] to PDK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250175 is the carrier-free version of ab180171 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250175 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13015 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
This data was developed using ab180171, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Mitochondrial Pyruvate dehydrogenase kinase 1 knockout HAP1 cell lysate (20 µg)
Lane 3: Human fetal heart tissue lysate (20 µg)
Lane 4: LnCaP cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab180171 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180171 was shown to specifically react with Mitochondrial Pyruvate dehydrogenase kinase 1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were examined. Wild-type and Mitochondrial Pyruvate dehydrogenase kinase 1 knockout samples were subjected to SDS-PAGE. ab180171 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)](https://www.abcam.com/ps/products/250/ab250175/Images/ab250175-398296-anti-pdk1-antibody-epr13015-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)All lanes : Anti-PDK1 antibody [EPR13015] - C-terminal (ab180171) at 1/10000 dilution
Lane 1 : Fetal heart lysate
Lane 2 : LNCaP cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDaThis data was developed using ab180171, the same antibody clone in a different buffer formulation.
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![Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)](https://www.abcam.com/ps/products/250/ab250175/Images/ab250175-3-benefits-of-recombinant-antibodies.png)
Anti-PDK1 antibody [EPR13015] - BSA and Azide free (ab250175)
