Anti-PDILT antibody (ab191851)
Key features and details
- Rabbit polyclonal to PDILT
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PDILT antibody -
Description
Rabbit polyclonal to PDILT -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Mouse PDILT aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: Q9DAN1
Synthetic peptide corresponding to Mouse PDILT aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: Q9DAN1 -
Positive control
- Testes extracts from wild-type and PDILT single knock-out mice; mouse testis tissue extract; mouse testicular section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDILT antibody (ab191851)
Immunohistochemical staining of paraffin embedded mouse testis tissue using ab191851 at a dilution of 1/1000. The secondary antibody was used at a dilution of 1/1000 and the sample was counter stained with DAPI.
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Western blot - Anti-PDILT antibody (ab191851)All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse sperm cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP anti-rabbit IgG at 1/10000 dilution
Predicted band size: 68 kDa
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Western blot - Anti-PDILT antibody (ab191851)All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1 : mouse brain tissue lysate at 30 µg
Lane 2 : mouse lung tissue lysate at 30 µg
Lane 3 : mouse heart tissue lysate at 30 µg
Lane 4 : mouse thymus tissue lysate at 30 µg
Lane 5 : mouse liver tissue lysate at 30 µg
Lane 6 : mouse spleen tissue lysate at 30 µg
Lane 7 : mouse kidney tissue lysate at 30 µg
Lane 8 : mouse muscle tissue lysate at 30 µg
Lane 9 : mouse ovary tissue lysate at 30 µg
Lane 10 : mouse testis tissue lysate at 30 µg
Lane 11 : mouse sperm lysate at 10 µg
Predicted band size: 68 kDa
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Western blot - Anti-PDILT antibody (ab191851)All lanes : Anti-PDILT antibody (ab191851) at 1/1000 dilution
Lane 1 : Testes extracts from wild-type (+/+) mice
Lane 2 : Testes extracts from PDILT single knock-out mice (+/-)
Lane 3 : Testes extracts from PDILT double knock-out mice (-/-)
Predicted band size: 68 kDaLower panel: detection of alpha tubulin was used as loading control.
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Immunoprecipitation - Anti-PDILT antibody (ab191851)Immunoprecipitation of PDILT protein from mouse testis extracts. Extracts (100 μg) of wild-type (+/+) and PDILT (-/-) knock out mouse testes were immunoprecipitated with ab191851 at 1/100 dilution and the precipitates were analyzed by western blotting using ab191851 at 1/1000 dilution.
1. Input. 2. Wild-type. 3. PDILT (-/-) knock out
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Immunocytochemistry/ Immunofluorescence - Anti-PDILT antibody (ab191851)Immunofluorescent analysis of paraformaldehyde-fixed, cryopreserved in graded 10-30% sucrose, and embedded in a TissueTek OCT compound mouse testicular sections labeling PDILT with ab191851 at 1/100 and as a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG antibody was used. Image at the top: mouse spermatocyte; image at the bottom mouse spermatid.