Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11099] to PDHA1 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDHA1 antibody [EPR11099] - BSA and Azide free
    See all PDHA1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11099] to PDHA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249188 is the carrier-free version of ab155096. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249188 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Western blot - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    All lanes : Anti-PDHA1 antibody [EPR11099] (ab155096) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : PDHA1 knockout HeLa whole cell lysate
    Lane 3 : HEK-293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    This data was developed using ab155096, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab155096 observed at 43 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab155096 was shown to recognize PDHA1 in wild-type HeLa cells as signal was lost at the expected MW in PDHA1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDHA1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab155096 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Western blot - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    All lanes : Anti-PDHA1 antibody [EPR11099] (ab155096) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 43 kDa



    This data was developed using ab155096, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Flow Cytometry - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)

    This data was developed using ab155096, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab155096 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab155096, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1 µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)

    This data was developed using ab155096, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PDHA1 with ab155096 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Immunocytochemistry - Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)

    This data was developed using ab155096, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HeLa cells labeling PDHA1 with ab155096 at 1/250 dilution.

  • Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)
    Anti-PDHA1 antibody [EPR11099] - BSA and Azide free (ab249188)

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