Anti-PDE4D (phospho S190) antibody (ab59212)
Key features and details
- Rabbit polyclonal to PDE4D (phospho S190)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDE4D (phospho S190) antibody
See all PDE4D primary antibodies -
Description
Rabbit polyclonal to PDE4D (phospho S190) -
Host species
Rabbit -
Specificity
ab59212 detects endogenous levels of PDE4D only when phosphorylated at serine 190. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human PDE4D around the phosphorylation site of serine 190 (R-E-SP-F-L).
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Positive control
- IHC-P: Human colon carcinoma tissue WB: K562 cell extracts treated with H2O2 (100uM, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab59212 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDE4D (phospho S190) antibody (ab59212)All lanes : Anti-PDE4D (phospho S190) antibody (ab59212) at 1/500 dilution
Lane 1 : K562 cell extract treated
with H2O2 (100uM, 30mins)
Lane 2 : K562 cell extract treated
with H2O2 (100uM, 30mins) with immunising peptide
Predicted band size: 91 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE4D (phospho S190) antibody (ab59212)ab59212, at 1/50 dilution, staining PDE4D in paraffin embedded human colon carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
