Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23546-160] to PD-L1 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free
    See all PD-L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23546-160] to PD-L1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Mouse splenocytes and A20 cells. ICC: A20 and RAW 264.7 cells.

  • General notes

    ab275384 is the carrier-free version of ab252436. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)
    Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

    This data was developed using ab252436, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of Mouse splenocyte cells labelling PD-L1 with ab252436 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable MHC II (I-A/I-E) positive cells.PD-L1 is highly expressed on DC cells as described in literature (PMID: 22902234).

  • Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)
    Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

    This data was developed using ab252436, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of  A20 (Mouse reticulum sarcoma B lymphocyte) cells labelling PD-L1 with ab252436 at 1/500 dilution (0.1ug) (Red), compared with a Rabbit monoclonal IgG (ab172730)  isotype contro l(Black), and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

  • Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)
    Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

    This data was developed using ab252436, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 cells labelling PD-L1 with ab252436 at 1/50 (10.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in A20 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)
    Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

    This data was developed using ab252436, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling PD-L1 with ab252436 at 1/50 (10.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in RAW 264.7 cell line after treatment with IFN-γ (100 ng/ml) for 24h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)
    Anti-PD-L1 antibody [EPR23546-160] - BSA and Azide free (ab275384)

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