Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-PCNA antibody [24/PCNA]
See all PCNA primary antibodies
Description

Mouse monoclonal [24/PCNA] to PCNA
Host species

Mouse

Tested Applications & Species

Application	Species
ICC	Rat Human
IHC-P	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, C6, RAW264.7, PC-12, NIH/3T3, Jurkat and A431 cell lysate. IHC-P: Human tonsil and colon tissue. Mouse and rat spleen tissue. ICC: HeLa, NIH/3T3 and C6 cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

24/PCNA
Isotype

IgG1
Research areas

Western blot - Anti-PCNA antibody [24/PCNA] (ab280088)

All lanes : Anti-PCNA antibody [24/PCNA] (ab280088) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

Exposure time: 26 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling PCNA with ab280088 at 1/5000 (0.215 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Nuclear staining on human tonsil.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunocytochemistry - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa cells labelling PCNA with ab280088 at 1/20 (53.7 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in HeLa cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Western blot - Anti-PCNA antibody [24/PCNA] (ab280088)

All lanes : Anti-PCNA antibody [24/PCNA] (ab280088) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 29 kDa

Exposure time: 6 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PCNA with ab280088 at 1/5000 (0.215 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on human colon.

The section was incubated with ab280088 for 30 mins at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PCNA with ab280088 at 1/5000 (0.215 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on mouse spleen.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PCNA with ab280088 at 1/5000 (0.215 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Nuclear staining on rat spleen.

The section was incubated with ab280088 for 30 minutes at room temperature and followed by rabbit specific IgG antibody ab125913 for 8 minutes. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 minutes.
Immunocytochemistry - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 cells labelling PCNA with ab280088 at 1/20 (53.7 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear and weak cytoplasmic staining in C6 cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Immunocytochemistry - Anti-PCNA antibody [24/PCNA] (ab280088)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling PCNA with ab280088 at 1/20 (53.7 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in NIH/3T3 cell line.

ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1: ab280088 at a 1/20 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Anti-PCNA antibody [24/PCNA] (ab280088)