Anti-PCID1 antibody (ab230105)
Key features and details
- Rabbit polyclonal to PCID1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PCID1 antibody
See all PCID1 primary antibodies -
Description
Rabbit polyclonal to PCID1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Orangutan, Xenopus tropicalis
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Immunogen
Recombinant full length protein corresponding to Human PCID1 aa 1 to the C-terminus.
Database link: Q7L2H7 -
Positive control
- WB: HeLa and A549 whole cell lysates; Human placenta tissue lysate; Mouse gonadal, stomach and brain tissue lysates. IP: HeLa whole cell lysate. IHC-P: Human pancreatic cancer and testis tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-PCID1 antibody (ab230105)All lanes : Anti-PCID1 antibody (ab230105) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : Mouse gonadal tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Mouse stomach tissue lysate
Lane 6 : Mouse brain tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28,43 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCID1 antibody (ab230105)Paraffin-embedded human pancreatic cancer tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCID1 antibody (ab230105)Paraffin-embedded human testis tissue stained for PCID1 using ab230105 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-PCID1 antibody (ab230105)PCID1 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg of ab230105. Western blot was performed from the immunoprecipitate using ab230105. HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
Lane 1: 1 µg control IgG instead of ab230105 in HeLa whole cell lysate .
Lane 2: ab230105 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
