Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12378] to PAR6 - N-terminal
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PAR6 antibody [EPR12378] - N-terminal
    See all PAR6 primary antibodies

  • Description

    Rabbit monoclonal [EPR12378] to PAR6 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PAR6 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NPB6

  • Positive control

    • HeLa, 293T, Jurkat, Y79 and fetal liver lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)
    Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)
    All lanes : Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Y79 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 37 kDa

  • Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)
    Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)

    Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)
    Anti-PAR6 antibody [EPR12378] - N-terminal (ab180159)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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