Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSPH antibody [001] - BSA and Azide free (ab276045)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [001] to PSPH - BSA and Azide free
  • Suitable for: WB, ICC, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PSPH antibody [001] - BSA and Azide free
    See all PSPH primary antibodies

  • Description

    Rabbit monoclonal [001] to PSPH - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human PSPH aa 1-225.
    Database link: P78330

  • Positive control

    • ICC: A549 cells. IP: K562 and HepG2 whole cell lysates. WB: MCF7, K562 and HepG2 whole cell lysates.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-PSPH antibody [001] (ab276045)
    Immunocytochemistry - Anti-PSPH antibody [001] (ab276045)

    Immunofluorescence staining of Human PSPH in A549 (human lung carcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with  ab276045 at 1/60 dilution at 4℃ overnight. Then cells were stained with the AlexaFluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-PSPH antibody [001] (ab276045)
    Immunoprecipitation - Anti-PSPH antibody [001] (ab276045)

    PSPH was immunoprecipitated using from: 
    Lane A:0.5 mg K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole Cell Lysate
    Lane B:0.5 mg HepG2 (human liver hepatocellular carcinoma cell line) whole Cell Lysate


    0.5 µL ab276045 and 15 µl of 50 % Protein G agarose.
    Primary antibody: ab276045, at 1/1000 dilution
    Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution

    Performed under reducing conditions.

  • Western blot - Anti-PSPH antibody [001] (ab276045)
    Western blot - Anti-PSPH antibody [001] (ab276045)
    All lanes : Anti-PSPH antibody [001] - BSA and Azide free (ab276045) at 1/500 dilution

    Lane 1 : MCF7 whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 25 kDa



    Developed using the Odyssey technique.


    Performed under reducing conditions.

  • Anti-PSPH antibody [001] - BSA and Azide free (ab276045)
    Anti-PSPH antibody [001] - BSA and Azide free (ab276045)

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