Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22853-61] to Pancreatic Polypeptide - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, mIHC
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free
    See all Pancreatic Polypeptide primary antibodies

  • Description

    Rabbit monoclonal [EPR22853-61] to Pancreatic Polypeptide - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, mIHCmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse pancreas tissue; rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; rat pancreas tissue. mIHC: Rat pancreas tissue.

  • General notes

    Ab256592 is the carrier-free version of ab255827. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256592 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22853-61

  • Isotype

    IgG

  • Research areas

Images

  • Multiplex immunohistochemistry - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Multiplex immunohistochemistry - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

    This data was developed using the same antibody clone in a different buffer formulation (ab255827).

    Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue.

    Panel A: Merged staining of anti-Pancreatic Polypeptide (ab255827, green), anti-Somatostatin 28 (ab111912, red) and DAPI (blue).

    Panel B: Anti-Pancreatic Polypeptide (green) stained on PP cells in rat pancreas islet.

    Panel C: Anti-Somatostatin 28 (red) stained on delta cells in rat pancreas islet.

    Panel D: Nuclear counter stain, DAPI (blue).

    Key protocol steps:  The section was incubated in two rounds of staining with ab255827 (1/20000 dilution) and ab111912 (1/2000 dilution) for 30 mins at room temperature. Each round was followed by tyramide signal amplification with the appropriate fluorophore. Heat mediated antigen retrieval was used (Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins after every round of antibody/fluorophore staining.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    A ready-to-use anti-Rabbit and Mouse Polymer HRP was used as a secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells in rat pancreas islet (PMID: 27270601) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab255827 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on PP cells in mouse pancreas islet (PMID: 27270601) is observed. Counter stained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab255827 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).

  • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

    Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). ab255827 and ab6995 are shown to stain PP cells and beta cells of rat pancreatic islets respectively (PMID: 24825414). The nuclear counter stain is DAPI (blue). Insulin is detected using ab6995, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

     

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).

  • Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Immunohistochemistry (Frozen sections) - Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

    Immunohistochemical analysis of frozen section of 4%PFA-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue labeling Pancreatic Polypeptide with ab255827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). ab255827 and ab6995 are shown to stain PP cells and beta cells of mouse pancreatic islet respectively (PMID: 24825414). The nuclear counter stain is DAPI (blue). Insulin is detected using ab6995, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255827).

  • Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)
    Anti-Pancreatic Polypeptide antibody [EPR22853-61] - BSA and Azide free (ab256592)

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