Antibodies, Proteins, Kits and Reagents for Life Science

241 228 ₸ Product size

100 µl 241 228 ₸

Order now and get it on Tuesday March 02, 2021

Anti-pan-myc antibody [EPR18863] (ab195207)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18863] to pan-myc
Suitable for: ICC/IF, WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-pan-myc antibody [EPR18863]
See all pan-myc primary antibodies
Description

Rabbit monoclonal [EPR18863] to pan-myc
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293 whole cell lysate transfected with n-Myc with GFP-tag; HEK-293 whole cell lysate transfected with c-Myc; HEK-293 whole cell lysate transfected with L-Myc with GFP-tag; RAW 264.7, K562, HeLa and HEK-293 whole cell lysates. ICC/IF: HeLa and RAW 264.7 cells. Flow Cyt: HeLa cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18863
Isotype

IgG
Research areas

Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/5000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with n-Myc with GFP-tag

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 83 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (ab195207)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-pan-myc antibody [EPR18863] (ab195207)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling pan-myc with ab195207 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab195207 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Flow Cytometry - Anti-pan-myc antibody [EPR18863] (ab195207)

Flow cytometry analysis of HeLa cells labelling pan-myc (red) with purified ab195207 at dilution of 1/60. The secondary antibody used was Alexa Fluor^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with c-Myc

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

All lanes : Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution

Lane 1 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
Lane 2 : HEK-293 whole cell lysate transfected with L-Myc with GFP-tag

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 83, 57 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

Anti-pan-myc antibody [EPR18863] (ab195207) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight for the mouse species is slightly higher than human Myc, this could be due to different degree of glycosylation.
Western blot - Anti-pan-myc antibody [EPR18863] (ab195207)

Anti-pan-myc antibody [EPR18863] (ab195207) at 1/2000 dilution + K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 49, 50, 40 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-pan-myc antibody [EPR18863] (ab195207)

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