Anti-PAICS/PAIS antibody (ab240743)
Key features and details
- Rabbit polyclonal to PAICS/PAIS
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAICS/PAIS antibody
See all PAICS/PAIS primary antibodies -
Description
Rabbit polyclonal to PAICS/PAIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human PAICS/PAIS aa 1-50. The exact sequence is proprietary. (NP_001072992.1).
Database link: P22234 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: PAICS/PAIS in HEK-293T whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
This product was previously labelled as PAICS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PAICS/PAIS antibody (ab240743)All lanes : Anti-PAICS/PAIS antibody (ab240743) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAICS/PAIS antibody (ab240743)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PAICS/PAIS using ab240743 at 1 µg/ml in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-PAICS/PAIS antibody (ab240743)PAICS/PAIS was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240743 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240743 at 0.4 µg/ml.
Lane 1: ab240743 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
