Anti-PABPN1 antibody (ab241477)
Key features and details
- Rabbit polyclonal to PABPN1
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PABPN1 antibody
See all PABPN1 primary antibodies -
Description
Rabbit polyclonal to PABPN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide within Human PABPN1 aa 1-50. The exact sequence is proprietary. (NP_004634.1).
Database link: Q86U42 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human stomach carcinoma tissue; Mouse teratoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241477 was affinity purified using an epitope specific to PABPN1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PABPN1 antibody (ab241477)All lanes : Anti-PABPN1 antibody (ab241477) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 3 seconds
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Immunoprecipitation - Anti-PABPN1 antibody (ab241477)PABPN1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241477 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241477 at 0.4 µg/ml.
Lane 1: ab241477 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 1 second.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (ab241477)Formalin-fixed, paraffin-embedded human stomach carcinoma tissue stained for PABPN1 using ab241477 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PABPN1 antibody (ab241477)Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for PABPN1 using ab241477 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
