Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17863-47] to p40 - DeltaNp63
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p40 - DeltaNp63 antibody [EPR17863-47]
    See all p40 - DeltaNp63 primary antibodies

  • Description

    Rabbit monoclonal [EPR17863-47] to p40 - DeltaNp63

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HaCaT and A431 whole cell lysate. IHC-P: Human lung squamous cell carcinoma tissue. Human breast and skin tissue. Mouse and rat skin tissue. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells. IP: HaCaT whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17863-47

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Western blot - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    All lanes : Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826) at 1/1000 dilution

    Lane 1 : HaCaT (human skin keratinocyte) whole cell lysate
    Lane 2 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 77 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa (PMID: 21631996).

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17615180, 26784942). 

    Exposure time: Lane  37 seconds; Lanes 2-3: 3 minutes.

  • Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    p40 - DeltaNp63 was immunoprecipitated from 0.35 mg HaCaT (Human skin keratinocyte) whole cell lysate 10µg with ab203826 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab203826 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HaCaT whole cell lysate 10µg.

    Lane 2: ab203826 IP in HaCaT whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203826 in HaCaT whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Flow Cytometry - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Flow Cytometry - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HaCaT (Human skin keratinocyte, Right) cells labeling p40 - DeltaNp63 with ab203826 at 1/400 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: HeLa. (PMID: 21631996).

  • Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human skin keratinocyte) HeLa (human cervix adenocarcinoma epithelial cell) cells labeling p40 - DeltaNp63 with ab203826 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in HaCaT cells.

    Negative control: HeLa (PMID: 21631996).

    ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the rat skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the mouse skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma and adenocarcinoma tissue labeling p40 - DeltaNp63 with ab203826 at a 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin.

    Nuclear staining on the squamous cell carcinoma (Panel A), negative staining on the adenocarcinoma (Panel B) is observed (PMID: 23486764).

    The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the basal cells of the human breast tissue is observed (PMID: 11839669; PMID: 28303349) The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the human skin is observed (PMID: 11839669). The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)
    Anti-p40 - DeltaNp63 antibody [EPR17863-47] (ab203826)

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