Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2141Y] to p130 - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-p130 antibody [EP2141Y] - BSA and Azide free
    See all p130 primary antibodies

  • Description

    Rabbit monoclonal [EP2141Y] to p130 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247453 is the carrier-free version of ab76234. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247453 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Western blot - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Anti-p130 antibody [EP2141Y] (ab76234) at 1/1000 dilution (unpurified) + Daudi cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size: 128 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76234, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Western blot - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Anti-p130 antibody [EP2141Y] (ab76234) at 1/1000 dilution (purified) + Daudi cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 128 kDa
    Observed band size: 130 kDa why is the actual band size different from the predicted?



    This data was developed using ab76234, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

    This data was developed using ab76234, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

    This data was developed using ab76234, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

    This data was developed using ab76234, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    This data was developed using ab76234, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    This data was developed using ab76234, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling p130 with unpurified ab76234 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    This data was developed using ab76234, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling p130 with purified ab76234 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    This data was developed using ab76234, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling p130 with unpurified ab76234 at 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Flow Cytometry - Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    This data was developed using ab76234, the same antibody clone in a different buffer formulation.Overlay histogram showing Jurkat cells stained with unpurified ab76234 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76234, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)
    Anti-p130 antibody [EP2141Y] - BSA and Azide free (ab247453)

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