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Anti-Oxytocin antibody [EPR20973] (ab212193)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20973] to Oxytocin
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Oxytocin antibody [EPR20973]
    See all Oxytocin primary antibodies

  • Description

    Rabbit monoclonal [EPR20973] to Oxytocin

  • Host species

    Rabbit

  • Specificity

    Specific express in pituitary tissue in WB and posterior lobe in IHC.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat pituitary tissue lysates. IHC-P: Mouse and rat hypothalamus tissues. IHC-Fr: Mouse and rat brain (Paraventricular nucleus) tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)
    Immunohistochemistry (Frozen sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)
    Immunohistochemistry (Frozen sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-Oxytocin antibody [EPR20973] (ab212193)
    Western blot - Anti-Oxytocin antibody [EPR20973] (ab212193)
    All lanes : Anti-Oxytocin antibody [EPR20973] (ab212193) at 1/1000 dilution

    Lane 1 : Mouse pituitary tissue lysate
    Lane 2 : Rat pituitary tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



     

    Exposure times: Lane 1: 3 seconds; Lane 2: 1 second.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oxytocin antibody [EPR20973] (ab212193)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Oxytocin antibody [EPR20973] (ab212193)
    Anti-Oxytocin antibody [EPR20973] (ab212193)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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