Anti-ORC1 antibody (ab85830)
Key features and details
- Rabbit polyclonal to ORC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ORC1 antibody
See all ORC1 primary antibodies -
Description
Rabbit polyclonal to ORC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region between residues 811 and 861 of human ORC1 (NP_004144.2)
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab85830 was affinity purified using an epitope specific to ORC1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ORC1 antibody (ab85830)All lanes : Anti-ORC1 antibody (ab85830) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunoprecipitation - Anti-ORC1 antibody (ab85830)Detection of Human ORC1 by Western Blot of Immunprecipitate.
All lanes: ab85830 at 1µg/ml staining ORC1 in HeLa whole cell lysates immunoprecipitated at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane) using the following antibodies:
Lane 1: ab85830
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
