Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Orai1 antibody [266.1] (ab175040)

Key features and details

  • Mouse monoclonal [266.1] to Orai1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Orai1 antibody [266.1]
    See all Orai1 primary antibodies

  • Description

    Mouse monoclonal [266.1] to Orai1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cynomolgus monkey

  • Immunogen

    Tissue, cells or virus. Cells expressing hOrai1—derived from C57BL6 mice.

  • Positive control

    • This antibody gave a positive signal in human and rat kidney, and human and rat testis

  • General notes

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai1 antibody [266.1] (ab175040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai1 antibody [266.1] (ab175040)
    IHC image of Orai1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab175040, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-Orai1 antibody [266.1] (ab175040)
    Western blot - Anti-Orai1 antibody [266.1] (ab175040)
    All lanes : Anti-Orai1 antibody [266.1] (ab175040) at 5 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Rat Kidney Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 32 kDa, 39 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Western blot - Anti-Orai1 antibody [266.1] (ab175040)
    Western blot - Anti-Orai1 antibody [266.1] (ab175040)
    Anti-Orai1 antibody [266.1] (ab175040) at 5 µg/ml + Testis (Rat) Tissue Lysate (ab4040) at 30 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?
    Additional bands at: 32 kDa, 39 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

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