Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ogg1 antibody [EPR4664(2)] (ab124741)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4664(2)] to Ogg1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Ogg1 antibody [EPR4664(2)]
    See all Ogg1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4664(2)] to Ogg1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Ogg1 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, JAR, Raji, NCCIT and HepG2 whole cell lysate (ab7900). Human cerebellum tissue lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Ogg1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab124741 observed at 38 kDa. Red - loading control, ab18058, observed at 124 kDa.
    ab124741 was shown to recognize Ogg1 when Ogg1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Ogg1 knockout samples were subjected to SDS-PAGE. ab124741 and ab18058 (loading control to vinculin) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/50000 dilution (purified)

    Lane 1 : JAR cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : NCCIT cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    Western blot - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    All lanes : Anti-Ogg1 antibody [EPR4664(2)] (ab124741) at 1/10000 dilution (unpurified)

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • OI-RD Scanning - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    OI-RD Scanning - Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Ogg1 antibody [EPR4664(2)] (ab124741)
    Anti-Ogg1 antibody [EPR4664(2)] (ab124741)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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