Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free
See all Oct6 primary antibodies
Description

Rabbit monoclonal [EPR24057-94] to Oct6 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC, IHC-Frmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat P0 brain, Rat P7 hippocampus, Mouse P1 brain, F9, NCCIT lysates. IHC-P: Human skin, Mouse cerebrum and Rat skin tissues. IHC-Fr: Mouse E14.5 embryonic, Rat E14.5 embryonic tissues. ICC: rat primary neuron, mouse primary neuron cellss. Flow Cyt: Mouse primary neuron, Rat primary neuron cells.
General notes
ab281842 is the carrier-free version of ab259952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab281842 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24057-94
Isotype

IgG

Western blot - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

All lanes : Anti-Oct6 antibody [EPR24057-94] (ab259952) at 1/1000 dilution

Lane 1 : Rat P0 brain tissue lysate
Lane 2 : Rat P7 hippocampus tissue lysate
Lane 3 : Rat lung tissue lysate
Lane 4 : Mouse P1 brain tissue lysate
Lane 5 : Mouse lung tissue lysate
Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
Lane 7 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: lung (PMID: 1979677).

Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of human skin. The section was incubated with ab259952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of rat skin. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat lung. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on rat E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

This data was developed using ab259952, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)