Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24057-94] to Oct6 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free
    See all Oct6 primary antibodies

  • Description

    Rabbit monoclonal [EPR24057-94] to Oct6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC, IHC-Frmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat P0 brain, Rat P7 hippocampus, Mouse P1 brain, F9, NCCIT lysates. IHC-P: Human skin, Mouse cerebrum and Rat skin tissues. IHC-Fr: Mouse E14.5 embryonic, Rat E14.5 embryonic tissues. ICC: rat primary neuron, mouse primary neuron cellss. Flow Cyt: Mouse primary neuron, Rat primary neuron cells.

  • General notes

    ab281842 is the carrier-free version of ab259952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281842 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24057-94

  • Isotype

    IgG

Images

  • Western blot - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Western blot - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    All lanes : Anti-Oct6 antibody [EPR24057-94] (ab259952) at 1/1000 dilution

    Lane 1 : Rat P0 brain tissue lysate
    Lane 2 : Rat P7 hippocampus tissue lysate
    Lane 3 : Rat lung tissue lysate
    Lane 4 : Mouse P1 brain tissue lysate
    Lane 5 : Mouse lung tissue lysate
    Lane 6 : F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate
    Lane 7 : NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 45 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?



    This data was developed using ab259952, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration:  5% NFDM/TBST

    Negative control: lung (PMID: 1979677).

    Exposure time: 3 minutes

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of human skin. The section was incubated with ab259952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse cerebrum. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on epithelial cells of rat skin. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat lung tissue labelling Oct6 with ab259952 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in rat lung. The section was incubated with ab259952 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

     

  • Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunohistochemistry (Frozen sections) - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat E14.5 embryonic tissue labeling Oct6 with ab259952 at 1/100 (5.22 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on rat E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Immunocytochemistry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Oct6 with ab259952 at 1/50 (10.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Flow Cytometry - Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

    This data was developed using ab259952, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Oct6 with ab259952 at 1/50 dilution (1ug)/(Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)
    Anti-Oct6 antibody [EPR24057-94] - BSA and Azide free (ab281842)

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