Anti-Oct4 antibody (ab137427)
Key features and details
- Rabbit polyclonal to Oct4
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Oct4 antibody
See all Oct4 primary antibodies -
Description
Rabbit polyclonal to Oct4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Cat, Pig, Monkey -
Immunogen
Recombinant fragment corresponding to Human Oct4 aa 1-182.
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Positive control
- Recombinant human Oct4 protein (ab134876) can be used as a positive control in WB. HeLa, Human embryonic stem cells (ESC) and Mouse ESC whole cell lysates; HeLa nuclear cell lysate; Human embryonic stem cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Affinity Purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Oct4 antibody (ab137427) at 1/5000 dilution + Mouse Embryonic Stem Cell whole cell lysate
at 20 µg
Predicted band size: 38 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:400 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:200 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed Human embryonic stem cells labelling Oct4 with ab137427 at 1:100 dilution. Lower image shows cells co-stained with DAPI.
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Anti-Oct4 antibody (ab137427) at 1/1000 dilution + HeLa nuclear cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Anti-Oct4 antibody (ab137427) at 1/1000 dilution + Human Embryonic Stem Cell whole cell lysate at 20 µg
Predicted band size: 38 kDa
10% SDS PAGE -
Flow cytometric analysis of Human Embryonic Stem cells labelling Oct4 with ab137427 at 1:50 dilution (green) or rabbit IgG (black).