Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76) - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free
    See all Nucleolin primary antibodies

  • Description

    Rabbit monoclonal [EPR8080-2] to Nucleolin (phospho T76) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Nucleolin when phosphorylated at threonine 76.

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Nucleolin (phospho T76). The exact sequence is proprietary.
    Database link: P19338

  • General notes

    Ab249459 is the carrier-free version of ab168363. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249459 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Western blot - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    All lanes : Anti-Nucleolin (phospho T76) antibody [EPR8080-2] (ab168363) at 1/1000 dilution

    Lane 1 : 293T cell lysates, untreated
    Lane 2 : 293T cell lysates, treated with Lambda Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 77 kDa



    This data was developed using ab168363, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)

    This data was developed using ab168363, the same antibody clone in a different buffer formulation.

    ab168363 showing +ve staining in Human ovarian carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)

    This data was developed using ab168363, the same antibody clone in a different buffer formulation.

    ab168363 showing +ve staining in Human kidney carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    This data was developed using ab168363, the same antibody clone in a different buffer formulation.ab168363 showing +ve staining in Human breast carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    This data was developed using ab168363, the same antibody clone in a different buffer formulation.ab168363 showing +ve staining in Human endometrial carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    This data was developed using ab168363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Nucleolin with ab168363 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    This data was developed using ab168363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human urinary bladder transitional cell carcinoma tissue labeling Nucleolin with ab168363 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Immunoprecipitation - Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    This data was developed using ab168363, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from 293 cell lysate using anti Phospho Nucleolin (pT76) ab158363 at 1/10 dilution.
  • Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)
    Anti-Nucleolin (phospho T76) antibody [EPR8080-2] - BSA and Azide free (ab249459)

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