Anti-NUCB1 antibody (ab154262)
Key features and details
- Rabbit polyclonal to NUCB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NUCB1 antibody
See all NUCB1 primary antibodies -
Description
Rabbit polyclonal to NUCB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment corresponding to Human NUCB1 aa 224-461.
Database link: Q02818 -
Positive control
- A549, HeLa, HepG2 and Mouse brain whole cell lysates; HeLa cells; Human NCIN87 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NUCB1 antibody (ab154262)Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Western blot - Anti-NUCB1 antibody (ab154262)Anti-NUCB1 antibody (ab154262) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUCB1 antibody (ab154262)Immunohistochemical analysis of paraffin-embedded Human NCIN87 xenograft tissue labeling NUCB1 with ab154262 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-NUCB1 antibody (ab154262)Immunofluorescent analysis of methanol-fixed HeLa cells labeling NUCB1 with ab154262 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
