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Anti-Npas4 antibody [S408-79] (ab242003)

Key features and details

  • Mouse monoclonal [S408-79] to Npas4
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Npas4 antibody [S408-79]
    See all Npas4 primary antibodies

  • Description

    Mouse monoclonal [S408-79] to Npas4

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Fusion protein corresponding to Rat Npas4 aa 550 to the C-terminus.
    Database link: Q8CJH6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain lysate. ICC/IF: SK-N-BE cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    S408-79

  • Isotype

    IgG1

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Npas4 antibody [S408-79] (ab242003)
    Immunocytochemistry/ Immunofluorescence - Anti-Npas4 antibody [S408-79] (ab242003)

    4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for Npas4 (green, C) using ab242003 at 1/100 dilution for 1 hour at room temperature in ICC/IF. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 1 hr at RT. Actin is detected with Phalloidin Texas Red F-Actin stain (red, B). The nuclear counterstain is DAPI (blue, A). (D) is a composite image.

  • Western blot - Anti-Npas4 antibody [S408-79] (ab242003)
    Western blot - Anti-Npas4 antibody [S408-79] (ab242003)
    Anti-Npas4 antibody [S408-79] (ab242003) at 1/1000 dilution + Rat brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/200 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa


    Exposure time: 6 minutes


    Primary incubation for 16 hours at 4°C.

    Secondary incubatin for 1 hr at RT.

    Blocked for 1 hr at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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