Anti-NOX3 antibody (ab81864)
Key features and details
- Rabbit polyclonal to NOX3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOX3 antibody
See all NOX3 primary antibodies -
Description
Rabbit polyclonal to NOX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human NOX3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101218) -
Positive control
- This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81864 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications DogApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
- Information by UniProt
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Database links
- Entrez Gene: 50508 Human
- Omim: 607105 Human
- SwissProt: Q9HBY0 Human
- Unigene: 247776 Human
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Alternative names
- EC 1.6.3.- antibody
- GP91 3 antibody
- GP91-3 antibody
see all
Images
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Western blot - Anti-NOX3 antibody (ab81864)All lanes : Anti-NOX3 antibody (ab81864) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends that this antibody is blocked with milk rather than BSA. -
Immunocytochemistry/ Immunofluorescence - Anti-NOX3 antibody (ab81864)ICC/IF image of ab81864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% MCF7 fixed (10 min) MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml
Protocols
Datasheets and documents
References (2)
ab81864 has been referenced in 2 publications.
- Liu Z et al. IFI6 depletion inhibits esophageal squamous cell carcinoma progression through reactive oxygen species accumulation via mitochondrial dysfunction and endoplasmic reticulum stress. J Exp Clin Cancer Res 39:144 (2020). PubMed: 32727517
- Plantinga TS et al. Rare NOX3 Variants Confer Susceptibility to Agranulocytosis During Thyrostatic Treatment of Graves' Disease. Clin Pharmacol Ther N/A:N/A (2017). Human . PubMed: 28486791
Images
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Western blot - Anti-NOX3 antibody (ab81864)All lanes : Anti-NOX3 antibody (ab81864) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends that this antibody is blocked with milk rather than BSA. -
Immunocytochemistry/ Immunofluorescence - Anti-NOX3 antibody (ab81864)ICC/IF image of ab81864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% MCF7 fixed (10 min) MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml
