Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [8G5] to NOTCH3 - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-NOTCH3 antibody [8G5] - BSA and Azide free
    See all NOTCH3 primary antibodies

  • Description

    Rat monoclonal [8G5] to NOTCH3 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ELISA
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG, T47D and Jurkat whole cell lysate.

  • General notes

    ab255839 is the carrier-free version of ab252845. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255839 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    All lanes : Anti-NOTCH3 antibody [8G5] (ab252845) at 1/1000 dilution

    Lane 1 : T47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 244 kDa



    This data was produced using the same clone in a different formulation, ab252845.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 30100605 and 24143218).
    Negative control: MDA-MB-231.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 70 seconds; Lanes 2-3: 3 minutes.

  • ELISA - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    ELISA - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)

    This data was produced using the same clone in a different formulation, ab252845.

    ab252845 used at 0-4000 ng/ml.

    Antigen (Human NOTCH3) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    Western blot - Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    Anti-NOTCH3 antibody [8G5] (ab252845) at 1/1000 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 244 kDa


    Exposure time: 3 minutes


    This data was produced using the same clone in a different formulation, ab252845.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 30100605 and 24143218).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)
    Anti-NOTCH3 antibody [8G5] - BSA and Azide free (ab255839)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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